NCERT : BIOLOGY : BIO TECHNOLOGY 2

QUESTIONS : 45

TIME : 25 MIN

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1) Which one of the following is now being commercially produced by biotechnological procedures? (NCERT XII:211)

A) Morphine

B) Nicotine

C) Quinine

D) Insulin

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2) What is antisense technology? (NCERT XII:209)

A) Production of somaclonal variants in tissue cultures

B) When a piece of RNA that is complementary in sequence is used to stop expression of a specific gene

C) A cell displaying a foreign antigen used for synthesis of antigens

D) RNA polymerase producing DNA

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3) Which of the following is not a component of downstream processing?

A) Purification

B) Separation

C) Preservation

D) Expression

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4) The process of separation and purification of expressed protein before marketing is called (NCERT XII:204)

A) bioprocessing

B) postproduction processing

C) downstream processing

D) upstream processing

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5) Which one of the following techniques made it possible to genetically engineer living organisms? (NCERT XII :194)

A) X-ray diffraction

B) Heavier isotope labelling

C) Hybridization

D) Recombinant DNA techniques

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6) Which one of the following is now being commercially produced by biotechnological procedures ___?

A) Nicotine

B) Quinine

C) Morphine

D) Insulin

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7) A gene whose expression helps to identify transformed cell is known as (NCERT XII:199)

A) plasmid

B) selectable marker.

C) vector

D) structural gene

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8)

A) B

B) A

C) C

D) D

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9) The two polypeptides of human insulin are linked together by (NCERT XII:211)

A) disulphide bridges

B) covalent bond

C) hydrogen bonds

D) phosphodiester bond.

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10) Which one of the following techniques made it possible to genetically engineer living organisms?

A) Heavier isotope labelling

B) X-ray diffraction

C) Recombinant DNA techniques

D) Hybridization

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11) The first clinical gene therapy was given for treating (NCERT XII:211)

A) diabetes mellitus

B) adenosine deaminase deficiency.

C) rheumatoid arthritis

D) chicken pox

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12) A ‘new’ variety of rice was patented by a foreign company, though such varieties have been present in India for a long time. This is related to ____?

A) Lerma Rojo

B) Sharbati Sonora

C) Co-667

D) Basmati

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13) Which body of the Government of India regulates GM research and safety of introducing GM organisms for public services?

A) Bio-safety committee

B) Research Committee on Genetic Manipulation

C) Genetic Engineering Approval Committee

D) Indian Council of Agricultural Research

14 / 45

14) Golden rice is a transgenic crop of the future with the following improved trait?

A) high lysine (essential amino acid) content

B) insect resistance

C) high vitamin-A content.

D) high protein content

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15) Genetic engineering is possible, because ___?

A) we can see DNA by electron microscope

B) we can cut DNA at specific sites by endonucleases like DNase I

C) the phenomenon of transduction in bacteria is well understood

D) restriction endonucleases purified from bacteria can be used in vitro

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16) The Taq polymerase enzyme is obtained from ?

A) Pseudomonas putida

B) Thermus aquaticus

C) Bacillus subtilis

D) Thiobacillus ferroxidans.

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17) A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using ?

A) EcoRI

B) Taq polymerase

C) polymerase III

D) ligase

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18) Which one of the following bacteria has found extensive use in genetic engineering work in plants?

A) Xanthomonas citri

B) Agrobacterium tumefaciens

C) Clostridium septicum

D) Bacillus coagulens

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19) Plasmid has been used as vector because __?

A) it can move between prokaryotic and eukaryotic cells

B) it has antibiotic resistance gene.

C) it is circular DNA which have capacity to join to eukaryotic DNA

D) both ends show replication

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20) Which vector can clone only a small fragment of DNA?

A) Cosmid

B) Yeast artificial chromosome

C) Plasmid

D) Bacterial artificial chromosome

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21) Bacillus thuringiensis forms protein crystals which contain insecticidal protein. This protein___?

A) does not kill the carrier bacterium which is itself resistant to this toxin.

B) binds with epithelial cells of midgut of the insect pest ultimately killing it

C) is coded by several genes including the gene cry

D) is activated by acid pH of the foregut of the insect pest

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22) Following statements describe the characteristics of the enzyme restriction endonuclease. Identify the incorrect statement.(NCERT XII:196)

A) The enzyme recognises a specific palindromic nucleotide sequence in the DNA.

B) The enzyme binds DNA at specific sites and cuts only one of the two strands.

C) The enzyme cuts the sugar-phosphate backbone at specific sites on each strand.

D) The enzyme cuts DNA molecule at identified position within the DNA.

23 / 45

23) Which one is a true statement regarding DNA polymerase used in PCR? (NCERT XII:203)

A) It remains active at high temperature.

B) It is used to ligate introduced DNA in recipient cells.

C) It serves as a selectable marker.

D) It is isolated from a virus.

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24) In India, the organisation responsible for assessing the safety of introducing genetically modified organisms for public use is (NCERT XII : 213)

A) Research Committee on Genetic Manipulation (RCGM)

B) Genetic Engineering Appraisal Committee (GEAC).

C) Indian Council of Medical Research (ICMR)

D) Council for Scientific and Industrial Research (CSIR)

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25) Gel electrophoresis is used for (NCERT XII:198)

A) construction of recombinant DNA by joining with cloning vectors

B) isolation of DNA molecules

C) separation of DNA fragments according to their size.

D) cutting of DNA into fragments

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26) Biolistics (gene-gun) is suitable for ___?

A) DNA fingerprinting.

B) transformation of plant cells

C) constructing recombinant DNA by joining with vectors

D) disarming pathogen vectors

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27) Choose the correct pair from the following?

A) Polymerases - Break the DNA into fragments

B) Nucleases - Separate the two strands of DNA

C) Ligases - Join the two DNA molecules

D) Exonucleases - Make cuts at specific positions within DNA

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28) Stirred-tank bioreactors have been designed for (NCERT XII:204)

A) addition of preservatives to the product

B) ensuring anaerobic conditions in the culture vessel

C) purification of the product

D) availability of oxygen throughout the process.

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29) Which one of the following is a case of wrong matching ?

A) Micropropagation – In vitro production of plants in large numbers

B) Somatic – Fusion of two hybridization diverse cells

C) Callus – Unorganized mass of cells produced in tissue culture

D) Vector DNA – Site for tRNA synthesis

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30) Maximum number of existing transgenic animals is of (NCERT XII:212)

A) fish

B) cow

C) pig.

D) mice

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31) Use of bioresources by multinational companies and organisations without authorisation from the concerned country and its people is called ?

A) biodegradation

B) bioexploitation.

C) bio-infringement

D) biopiracy

32 / 45

32) What is antisense technology ___?

A) Production of somaclonal variants in tissue cultures

B) When a piece of RNA that is complementary in sequence is used to stop expression of a specific gene

C) A cell displaying a foreign antigen used for synthesis of antigens

D) RNA polymerase producing DNA

33 / 45

33) Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes? (NCERT XII:200)

A) λ phage

B) Ti plasmid

C) pBR322

D) Retrovirus

34 / 45

34) Human insulin is being commercially produced from a transgenic species of __?

A) Mycobacterium

B) Saccharomyces

C) Rhizobium

D) Escherichia

35 / 45

35) Restriction endonucleases are

A) used for in vitro DNA synthesis

B) synthesized by bacteria

C) used in genetic engineering

D) present in mammalian cells for degradation of DNA.

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36) The linking of antibiotic resistance gene with the plasmid vector became possible with (NCERT XII:197)

A) DNA polymerase

B) exonucleases

C) endonucleases

D) DNA ligase

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37) In gel electrophoresis, separated DNA fragments can be visualized with the help of ?

A) acetocarmine in UV radiation

B) acetocarmine in bright blue light

C) ethidium bromide in infrared radiation.

D) ethidium bromide in UV radiation

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38) The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of (NCERT XII:200)

A) non-recombinant bacteria containing beta galactosidase

B) insertional inactivation of alpha galactosidase in recombinant bacteria

C) inactivation of glycosidase enzyme in recombinant bacteria

D) insertional inactivation of alpha galactosidase in non-recombinant bacteria.

39 / 45

39) Restriction endonucleases are

A) used for in vitro DNA synthesis

B) used in genetic engineering

C) synthesized by bacteria

D) present in mammalian cells for degradation of DNA.

40 / 45

40) Genetic engineering is possible, because (NCERT XII:194)

A) we can cut DNA at specific sites by endonucleases like DNase I

B) restriction endonucleases purified from bacteria can be used in vitro

C) we can see DNA by electron microscope

D) the phenomenon of transduction in bacteria is well understood

41 / 45

41) Which of the following is not a feature of the plasmids?

A) Circular structure

B) Transferable

C) Single-stranded

D) Independent replication

42 / 45

42) The linking of antibiotic resistance gene with the plasmid vector became possible with __?

A) DNA ligase

B) endonucleases

C) exonucleases

D) DNA polymerase

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43) Two bacteria found to be very useful in genetic engineering experiments are

A) Rhizobium and Diplococcus

B) Nitrosomonas and Klebsiella

C) Nitrobacter and Azotobacter

D) Escherichia and Agrobacterium.

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44)

A) D

B) A

C) C

D) B

45 / 45

45) The process of RNA interference (RNAi) has been used in the development of plants resistant to __?

A) fungi

B) viruses

C) insects.

D) nematodes

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NCERT : BIOLOGY : BIO TECHNOLOGY 2

TIME : 25 MIN

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