NCERT : BIOLOGY : BIO TECHNOLOGY 2

QUESTIONS : 45

TIME : 25 MIN

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1) The two polypeptides of human insulin are linked together by (NCERT XII:211)

A) covalent bond

B) phosphodiester bond.

C) hydrogen bonds

D) disulphide bridges

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2) Which of the following is not a component of downstream processing?

A) Purification

B) Preservation

C) Separation

D) Expression

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3) The Taq polymerase enzyme is obtained from ?

A) Bacillus subtilis

B) Thermus aquaticus

C) Pseudomonas putida

D) Thiobacillus ferroxidans.

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4) Which one is a true statement regarding DNA polymerase used in PCR? (NCERT XII:203)

A) It is isolated from a virus.

B) It remains active at high temperature.

C) It serves as a selectable marker.

D) It is used to ligate introduced DNA in recipient cells.

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5) Which vector can clone only a small fragment of DNA?

A) Cosmid

B) Plasmid

C) Yeast artificial chromosome

D) Bacterial artificial chromosome

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6) Biolistics (gene-gun) is suitable for ___?

A) constructing recombinant DNA by joining with vectors

B) disarming pathogen vectors

C) DNA fingerprinting.

D) transformation of plant cells

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7) A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using ?

A) Taq polymerase

B) EcoRI

C) polymerase III

D) ligase

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8) Following statements describe the characteristics of the enzyme restriction endonuclease. Identify the incorrect statement.(NCERT XII:196)

A) The enzyme recognises a specific palindromic nucleotide sequence in the DNA.

B) The enzyme cuts DNA molecule at identified position within the DNA.

C) The enzyme cuts the sugar-phosphate backbone at specific sites on each strand.

D) The enzyme binds DNA at specific sites and cuts only one of the two strands.

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9) Gel electrophoresis is used for (NCERT XII:198)

A) separation of DNA fragments according to their size.

B) construction of recombinant DNA by joining with cloning vectors

C) isolation of DNA molecules

D) cutting of DNA into fragments

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10) A ‘new’ variety of rice was patented by a foreign company, though such varieties have been present in India for a long time. This is related to ____?

A) Sharbati Sonora

B) Co-667

C) Lerma Rojo

D) Basmati

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11) The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of (NCERT XII:200)

A) inactivation of glycosidase enzyme in recombinant bacteria

B) non-recombinant bacteria containing beta galactosidase

C) insertional inactivation of alpha galactosidase in recombinant bacteria

D) insertional inactivation of alpha galactosidase in non-recombinant bacteria.

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12) Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes? (NCERT XII:200)

A) Retrovirus

B) λ phage

C) Ti plasmid

D) pBR322

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13) The process of RNA interference (RNAi) has been used in the development of plants resistant to __?

A) viruses

B) insects.

C) nematodes

D) fungi

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14) Stirred-tank bioreactors have been designed for (NCERT XII:204)

A) addition of preservatives to the product

B) purification of the product

C) availability of oxygen throughout the process.

D) ensuring anaerobic conditions in the culture vessel

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15) Two bacteria found to be very useful in genetic engineering experiments are

A) Nitrobacter and Azotobacter

B) Escherichia and Agrobacterium.

C) Nitrosomonas and Klebsiella

D) Rhizobium and Diplococcus

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16) Human insulin is being commercially produced from a transgenic species of __?

A) Rhizobium

B) Escherichia

C) Mycobacterium

D) Saccharomyces

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17) Which one of the following is now being commercially produced by biotechnological procedures? (NCERT XII:211)

A) Nicotine

B) Insulin

C) Morphine

D) Quinine

18 / 45

18) Genetic engineering is possible, because ___?

A) we can cut DNA at specific sites by endonucleases like DNase I

B) the phenomenon of transduction in bacteria is well understood

C) we can see DNA by electron microscope

D) restriction endonucleases purified from bacteria can be used in vitro

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19) In gel electrophoresis, separated DNA fragments can be visualized with the help of ?

A) ethidium bromide in UV radiation

B) acetocarmine in bright blue light

C) acetocarmine in UV radiation

D) ethidium bromide in infrared radiation.

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20) Which of the following is not a feature of the plasmids?

A) Transferable

B) Single-stranded

C) Circular structure

D) Independent replication

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21) A gene whose expression helps to identify transformed cell is known as (NCERT XII:199)

A) plasmid

B) selectable marker.

C) vector

D) structural gene

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22) Which body of the Government of India regulates GM research and safety of introducing GM organisms for public services?

A) Bio-safety committee

B) Indian Council of Agricultural Research

C) Genetic Engineering Approval Committee

D) Research Committee on Genetic Manipulation

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23) The first clinical gene therapy was given for treating (NCERT XII:211)

A) diabetes mellitus

B) chicken pox

C) adenosine deaminase deficiency.

D) rheumatoid arthritis

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24)

A) B

B) C

C) A

D) D

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25) Genetic engineering is possible, because (NCERT XII:194)

A) the phenomenon of transduction in bacteria is well understood

B) we can cut DNA at specific sites by endonucleases like DNase I

C) we can see DNA by electron microscope

D) restriction endonucleases purified from bacteria can be used in vitro

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26) Restriction endonucleases are

A) used in genetic engineering

B) present in mammalian cells for degradation of DNA.

C) synthesized by bacteria

D) used for in vitro DNA synthesis

27 / 45

27) The linking of antibiotic resistance gene with the plasmid vector became possible with (NCERT XII:197)

A) exonucleases

B) endonucleases

C) DNA polymerase

D) DNA ligase

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28) Which one of the following techniques made it possible to genetically engineer living organisms?

A) Recombinant DNA techniques

B) Hybridization

C) X-ray diffraction

D) Heavier isotope labelling

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29) Which one of the following techniques made it possible to genetically engineer living organisms? (NCERT XII :194)

A) Recombinant DNA techniques

B) Heavier isotope labelling

C) Hybridization

D) X-ray diffraction

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30) What is antisense technology? (NCERT XII:209)

A) Production of somaclonal variants in tissue cultures

B) A cell displaying a foreign antigen used for synthesis of antigens

C) RNA polymerase producing DNA

D) When a piece of RNA that is complementary in sequence is used to stop expression of a specific gene

31 / 45

31) Which one of the following bacteria has found extensive use in genetic engineering work in plants?

A) Agrobacterium tumefaciens

B) Clostridium septicum

C) Bacillus coagulens

D) Xanthomonas citri

32 / 45

32) Choose the correct pair from the following?

A) Nucleases - Separate the two strands of DNA

B) Polymerases - Break the DNA into fragments

C) Ligases - Join the two DNA molecules

D) Exonucleases - Make cuts at specific positions within DNA

33 / 45

33) Which one of the following is a case of wrong matching ?

A) Micropropagation – In vitro production of plants in large numbers

B) Vector DNA – Site for tRNA synthesis

C) Callus – Unorganized mass of cells produced in tissue culture

D) Somatic – Fusion of two hybridization diverse cells

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34) The process of separation and purification of expressed protein before marketing is called (NCERT XII:204)

A) upstream processing

B) downstream processing

C) bioprocessing

D) postproduction processing

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35) In India, the organisation responsible for assessing the safety of introducing genetically modified organisms for public use is (NCERT XII : 213)

A) Genetic Engineering Appraisal Committee (GEAC).

B) Indian Council of Medical Research (ICMR)

C) Research Committee on Genetic Manipulation (RCGM)

D) Council for Scientific and Industrial Research (CSIR)

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36) The linking of antibiotic resistance gene with the plasmid vector became possible with __?

A) DNA ligase

B) exonucleases

C) endonucleases

D) DNA polymerase

37 / 45

37)

A) D

B) B

C) C

D) A

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38) Maximum number of existing transgenic animals is of (NCERT XII:212)

A) pig.

B) cow

C) mice

D) fish

39 / 45

39) Plasmid has been used as vector because __?

A) it is circular DNA which have capacity to join to eukaryotic DNA

B) it can move between prokaryotic and eukaryotic cells

C) both ends show replication

D) it has antibiotic resistance gene.

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40) Use of bioresources by multinational companies and organisations without authorisation from the concerned country and its people is called ?

A) biopiracy

B) bio-infringement

C) bioexploitation.

D) biodegradation

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41) What is antisense technology ___?

A) Production of somaclonal variants in tissue cultures

B) RNA polymerase producing DNA

C) When a piece of RNA that is complementary in sequence is used to stop expression of a specific gene

D) A cell displaying a foreign antigen used for synthesis of antigens

42 / 45

42) Golden rice is a transgenic crop of the future with the following improved trait?

A) high protein content

B) insect resistance

C) high lysine (essential amino acid) content

D) high vitamin-A content.

43 / 45

43) Which one of the following is now being commercially produced by biotechnological procedures ___?

A) Quinine

B) Nicotine

C) Morphine

D) Insulin

44 / 45

44) Bacillus thuringiensis forms protein crystals which contain insecticidal protein. This protein___?

A) does not kill the carrier bacterium which is itself resistant to this toxin.

B) is activated by acid pH of the foregut of the insect pest

C) is coded by several genes including the gene cry

D) binds with epithelial cells of midgut of the insect pest ultimately killing it

45 / 45

45) Restriction endonucleases are

A) synthesized by bacteria

B) used in genetic engineering

C) used for in vitro DNA synthesis

D) present in mammalian cells for degradation of DNA.

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NCERT : BIOLOGY : BIO TECHNOLOGY 2

TIME : 25 MIN

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